Differentiating the distinct roles of soluble and bound amyloid-β on microglial accumulation in Alzheimer's disease

Progressive microglial accumulation at amyloid-β (Aβ) plaques is a well-established signature of the pathology of Alzheimer's disease, but how and why microglia accumulate in the vicinity of Aβ plaques is unknown. To understand the distinct roles of Aβ on microglial accumulation, we quantified microglial responses to week-long lasting gradients of soluble Aβ and patterns of surface-bound Aβ in microfluidic platforms.

3D in vitro blood-brain-barrier model in planar microfluidics

Our BBB model is constructed in a tube form and implemented in a planar microfluidic platform. Particularly, the model is designed to reconstitute the penetration of white blood cells through destructed tight junctions in brain endothelial cells, driven by gradients of chemoattractants during neuro-inflammatory diseases.